Figure 5
Figure 5. Antigen binding by tumor cells of patient 1152 correlates positively with BCR expression. A single-cell suspension of the tumor biopsy was analyzed by flow cytometry to assess antigen binding by individual cells. Recombinant myoferlin with an HA tag or fused to mouse IgG2a FC was used to stain the cells. Antigen binding was detected with goat anti-HA or goat anti–mouse IgG2a. Lysate from untransfected 293T cells served as a negative control (UT). Cells are gated on CD3−CD20+ B cells; tumor B cells and nontumor B cells were identified with CD20hiIgM− and CD20intIgM+ gates, respectively.

Antigen binding by tumor cells of patient 1152 correlates positively with BCR expression. A single-cell suspension of the tumor biopsy was analyzed by flow cytometry to assess antigen binding by individual cells. Recombinant myoferlin with an HA tag or fused to mouse IgG2a FC was used to stain the cells. Antigen binding was detected with goat anti-HA or goat anti–mouse IgG2a. Lysate from untransfected 293T cells served as a negative control (UT). Cells are gated on CD3−CD20+ B cells; tumor B cells and nontumor B cells were identified with CD20hiIgM− and CD20intIgM+ gates, respectively.

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