Myoferlin stimulates phosphorylation of S6 ribosomal protein in tumor cells. A single-cell suspension of the tumor biopsy was stimulated with 100 μL of detergent-adsorbed lysate of either untransfected 293T cells (UT), or 293T cells transfected to express recombinant myoferlin containing an HA tag or myoferlin fused to mouse IgG2a Fc. A total of 10 μg/mL of goat anti-IgG and IgM was used as positive control for BCR signaling for tumor and nontumor B cells, respectively. Cells were stimulated for 45 minutes at 37°C. Cells were then fixed with 1.6% paraformaldehyde and permeabilized with methanol. Cells were stained for expression of CD3, CD20, and phosphorylated S6 ribosomal proteins. Tumor and nontumor B cells were identified with CD3⁻CD20^hi and CD3⁻CD20^int gates, respectively. Values adjacent to histograms indicate median fluorescent intensities.