Results from IC Flow-FISH. (A,D) Scatter plots of forward and side scatter allowed gating of lymphocytes and monocytes distinct from particulate debris in donor and patient samples. (B,E) Scatter plots of FSC-A versus chromosome 7 probe signal gave vertical separation of (top) monocyte and (bottom) lymphocyte populations based on size. In samples with monosomy 7, some or all monocytes showed lower chromosome 7 probe binding. A small third population, rightmost and unshaded in the monosomy 7 sample plot, was frequently present in patient and donor samples. This population appeared as 2-cell clumps or G2M cells after FACS sorting and fluorescence microscopy (separate sample, K.K., J.W., unpublished data, March 2012). (C,F) Histograms of the raw data corresponding to the blue-shaded regions in panels B,E with a linear axis for probe binding.