FACS isolation of monosomy 7 cells after IC Flow-FISH. Monosomy 7 cells (A) and normal cells (B) from PBMCs (frozen for 6 weeks after drawing) were sorted during IC Flow-FISH, stained with 4,6-diamidino-2-phenylindole, and photographed with fluorescence microscopy. Cells were sorted on a BD FACSAria II cell sorter; pictures were taken at room temperature on an Axio Imager D1 (Carl Zeiss Imaging Systems) with 20×/1.4 objective and processed by AxioVision Version 4.6.3.0 (Carl Zeiss Imaging Systems).