Figure 3
Figure 3. Gfi1 and Gfi1b are expressed at the onset of hematopoiesis in vivo. (A) FACS analysis of CD41+GFP− (wild type; top panels), CD41+Gfi1-GFP+ (Gfi1+/GFP; middle panels), and CD41+Gfi1b-GFP+ (Gfi1b+/GFP; bottom panels) cells for expression of KIT, TIE2, and FLK1 cell-surface markers. Each dot plot corresponds to the concatenation of the data from 3 individual E8.5 embryos of the indicated genotype using the concatenation function of FlowJo. Numbers indicate percentage. (B) Merged fluorescence and bright field images of late headfold E8.5 Gfi1b+/GFP embryos (5× objective). n indicates node; and ng, neural groove.

Gfi1 and Gfi1b are expressed at the onset of hematopoiesis in vivo. (A) FACS analysis of CD41+GFP− (wild type; top panels), CD41+Gfi1-GFP+ (Gfi1+/GFP; middle panels), and CD41+Gfi1b-GFP+ (Gfi1b+/GFP; bottom panels) cells for expression of KIT, TIE2, and FLK1 cell-surface markers. Each dot plot corresponds to the concatenation of the data from 3 individual E8.5 embryos of the indicated genotype using the concatenation function of FlowJo. Numbers indicate percentage. (B) Merged fluorescence and bright field images of late headfold E8.5 Gfi1b+/GFP embryos (5× objective). n indicates node; and ng, neural groove.

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