SGI-1776 triggers MM cell death. (A) MM.1S cells were treated with SGI-1776 (3μM) for 24 hours. Total protein lysates were subjected to immunoblotting with the indicated Abs. (B) MM cell lines were treated with SGI-1776 at indicated the concentrations for 48 hours, followed by assessment of cell viability using the MTT assay. Data presented are means ± SD of 3 independent experiments. (C) Purified CD138⁺ cells were treated with SGI-1776 at the indicated concentrations for 48 hours, and cell viability was measured using the CellTiter-Glo assay. Data are presented as means ± SD of triplicates. (D) Leukemia and lymphoma cell lines were treated with SGI-1776 at the indicated concentrations for 48 hours and cell viability was measured with the MTT assay. Data are presented as means ± SD of 3 independent experiments. (E) MM.1s cells were treated with vehicle or SGI-1776 at 3 and 5μM for 24 hours. Cells were harvested and analyzed for apoptosis by annexin V-FITC/propidium iodide double staining. Data shown are representative of 3 experiments.