Figure 5
![Figure 5. Endogenous SLC35D3 and STX13 cofractionate in subcellular fractions of G1ME cells. Gata-1–transduced G1ME cells were lysed by Dounce homogenization, and membranes were fractionated by Percoll gradient centrifugation. Individual fractions were analyzed by Western blotting with Abs to endogenous SLC35D3 or to MRP4 (a marker of DGs), STX13 (a marker of early endosomes [EE]) or vWF (a marker of α granules [αGs]. Note that the peak of reactivity for SLC35D3 overlaps best with STX13, supporting early endosome localization, and a small trail cofractionates with MRP-4.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/120/2/10.1182_blood-2011-11-389551/4/zh89991293390005.jpeg?Expires=1735562471&Signature=mHv8aXRGZFMc65qWz6kOmTcUftJc8Aw5cP13ntrYGVNWlOSJCDEhVzQtRLHcN7KMo94~54mx5K0DwN78Uw2GXbJkyxlAQmVTSfASBLON87LWLqHvHs3XI3hTCfbhOjjBeUJk21lP0~GlldUZi0grC4KY8xuXTs5geDnNM4hDyr-yrIHsI0sjaJI~bTO4h5Go9qm7gHD33M0k4HSAkUPz6hCC0n7Piau1NbTzBGX26VzT0~pcvQTM4w0u~2xVjlVokIXeMY46ltwWGufGvu~hQeCDQQvlnue9wNwJVa2r6CYd43e1bPOw09hnahHgbsfwR-eefDJyZhjlwOr5sNwo8g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Endogenous SLC35D3 and STX13 cofractionate in subcellular fractions of G1ME cells.Gata-1–transduced G1ME cells were lysed by Dounce homogenization, and membranes were fractionated by Percoll gradient centrifugation. Individual fractions were analyzed by Western blotting with Abs to endogenous SLC35D3 or to MRP4 (a marker of DGs), STX13 (a marker of early endosomes [EE]) or vWF (a marker of α granules [αGs]. Note that the peak of reactivity for SLC35D3 overlaps best with STX13, supporting early endosome localization, and a small trail cofractionates with MRP-4.
