Rab32 and Rab38 colocalize with the late endocytic marker Rab7a but not with the vacuolar early endosome and recycling endosome marker Rab5a. Cherry-Rab32 and Cherry-Rab38 were cotransfected with GFP-Rab5a or GFP-Rab7a in MEG-01 cells. Confocal fluorescence microscopy images of live cells together with the corresponding fluorescence intensity line scan graphs are shown for each experiment. The white lines in the merge panels indicate the portions of the cells where fluorescence intensities for both the red and green channels were measured. (A,C) Neither Rab32 nor Rab38 colocalizes significantly with Rab5a, a marker of recycling vesicles/early endosomes (MOC = 0.16 ± 0.02, n = 13 cells and MOC = 0.14 ± 0.01, n = 7 cells, respectively). (B,D) Consistent with Rab32 and Rab38 being present in immature DGs, both proteins colocalize with Rab7a, a marker of late endosomal compartments that is also present in other LROs such as melanosomes and lamellar bodies (MOC = 0.52 ± 0.02, n = 7 cells and MOC = 0.42 ± 0.03, n = 7 cells, respectively).