Schematic presentation of the changes in subclonal architecture over time. (A,D,F) Schematic representation of the subclonal hierarchy for patients CLL003 (A), CLL077 (D), and CLL006 (F). Tumor subclones (red circles) are mapped to each stage and extrapolated back to the origin. The number beside each circle shows the percentage of cells calculated using mutant allele frequencies. Colored boxes denote mutation profile groups (Figure 1D, supplemental Figure 3). (B-C,E,G-H) Graphic illustration of absolute cell numbers for each subclone at all stages for patients CLL003 (B-C), CLL077 (E), and CLL006 (G-H). Plots are expanded for stage c of patient CLL003 (C) and stage c of patient CLL006 (H).