Induction of MKs from transfected adult HDFs. Adult HDFs transfected with p45NF-E2, Maf G, and Maf K were cultured in MKLI medium, and large-sized cells of the cultured cells were isolated by a 2-step BSA gradient. Representative data of CD41 (A) and CD41 and CD42b (B) expression on the large-sized cells from 3 repeats. (C) DNA ploidy analysis on HDFs transfected with p45NF-E2, Maf G, and Maf K (N = 3; mean ± SEM %: 2N, 60.7 ± 4.8; 4N, 19.5 ± 1.8; 8N, 8.7 ± 1.0; and ≥ 16N, 11.0 ± 3.0).