iMK-derived platelets into thrombus formation on a collagen chip. Representative studies of FITC-anti–human CD41 antibody-labeled blood samples from iMK-infused thrombocytopenic NOG mice perfused on a collagen-coated chip under flow condition (1000 seconds−1) for 10 minutes. (A) Collagen-coated chip before perfusion. (B) Thrombi on the collagen-coated chip after the perfusion. (C) iMK-derived platelets labeled with FITC anti–human CD41 antibody are shown in bright green fluorescence and are incorporated into the thrombi. (D) Similar to panel B but after human platelets had been infused into recipient mice. (E) Whole blood samples from irradiated and thrombocytopenic NOG mice with no infused human cells were perfused on the collagen-coated chip under flow condition (1000 seconds−1) for 10 minutes. (F) Similar to panel C but for human platelets. Original magnification, ×100, and a 50 μm bar is shown in bottom right corner of images.
