LN anatomic distribution of productively infected cells in RMs, humans, and SMs. (A) Quantitation of the number of SIV/HIV vRNA+ cells/mm2 in LNs of chronically infected RMs, humans, and SMs. (B) Frequency of SIV/HIV vRNA+ cells located within B-cell follicles in LNs of chronically infected RMs, humans, and SMs. (C) The percentage of LN area that consists of B-cell follicles. (D) The percentage LN area of Gag+ (SIVp17+ or HIVp24+) FDCs in chronically infected RMs, humans, and SMs. (E) Combination SIV/HIV in situ hybridization and CD20 IHC showing the relative frequency of SIV/HIV vRNA+ cells located within B-cell follicles (arrowheads) and in the extrafollicular T-cell zone (arrows) in a representative RM, human, and SM at low magnification (top panels) and high magnification (bottom panel). Scale bars represent 50 μm. (F) SIVp17/HIVp24 Gag IHC showing the relative amount of FDC trapped SIV/HIV from 2 chronically infected RMs, humans and SMs. Note the dramatic variability in FDC trapping in SM compared with RM and human. Scale bars represent 50 μm. Horizontal lines represent the median. P values are based on the Mann-Whitney test.
