HSC number and long-term repopulating activity are normal in N-cadherin–deleted mice. (A) The frequency of KLS cells (n = 5). (B) The frequency of KLS CD41⁻ CD48⁻ CD150⁺ (KLS SLAM) cells (n = 5). (C) Competitive repopulation assays were performed, and the number of repopulating units was calculated up to 7.5 months after transplantation. Data are representative of 2 independent transplants with n = 4 or 5 mice per group per experiment. (D) Competitive repopulation assays were repeated with N-cadherin–deleted or control cells, and then secondary transplantation was performed after 12 weeks. Shown is the percentage of donor cells in the primary recipients (at 12 weeks) or secondary recipients (at 8 weeks, n = 5). (E-F) Bone marrow cells expressing the Ly5.1 locus were transplanted into irradiated N-cadherin–deleted or control mice. Donor engraftment (E) and lineage distribution (F) in the peripheral blood 38 weeks after transplantation (n = 4). Data are the mean ± SEM.