Correlation of tumor pSTAT3 and Myc expression with pretreatment serum cytokines in the same patients treated on N0489. (A) Correlation of serum cytokine levels with pSTAT3 and Myc detection by IHC. (B) The serum-starved DHL2 cell line was treated with recombinant human IL-10, G-CSF, or TNF-α (R&D Systems) for various time intervals and subjected to Western blotting for pSTAT3 (tyrosine) antibody.