Shifted cell cycle status and increased apoptosis in TGFβ-hypersensitive cells. (A) FACS analysis of unsorted transduced CD34⁺ CB cells cultured under serum-free conditions for 5-6 days. (B) Average percentages of cell cycle status in GFP⁺ cells without or (C) with inhibitor SB431542, ± SEM (n = 4). (D) Representative FACS plots and (E) average percentages of GFP⁺ cells in early apoptosis (annexin V⁺7–AAD⁻) with or without inhibitor SB431542, ± SEM (n = 5). *P ≤ .05 (paired Student t test or the Mann-Whitney test).