Figure 6
Blocking of DCIR and BDCA-2 restores production of IFN-α and IFN-λ in pDCs exposed to HCVcc particles. (A) Experimental flowchart. pDCs were saturated with 20 μg/mL of Fab fragments of DCIR and/or BDCA-2 antibody at 4°C. Fab-saturated pDCs were then exposed to HCVcc particles (MOIHCV RNA = 100) and stimulated with CpG-A. (B-C) Production of IFN-α (B) or IFN-λ (C) in cell-free supernatant was determined by ELISA 20 hours after stimulation. Data are mean ± SEM of 3 independent experiments with pDCs from different donors. **P < .01.
