Exposure of whole blood samples to HIV-1 results in increased surface expression of TF on nonclassic (CD14⁺CD16⁺⁺) monocytes, but not on the intermediate (CD14⁺⁺CD16⁺ CD14⁺CD16⁺) or CD14⁺⁺CD16⁻ monocytes. Whole blood was obtained from HIV-1–uninfected subjects and exposed to LPS (50 ng/mL), imiquimod (5 μg/mL), single-stranded PolyU complexed with the cationic lipid Lyovec^R (ssPolyU; 10 μg/mL), or AT-2–inactivated HIV-1 (X4 or R5 tropic, 150 ng/mL) for 3 hours. Surface expression of TF was measured on monocyte subsets by flow cytometry. Exposure to LPS resulted in a significant increase in TF on all monocyte subsets, but exposure to imiquimod, ssPolyU, or HIV-1 resulted in increased TF expression on only the CD14⁺CD16⁺⁺ subset.