CD4⁺, CXCR3⁺, and EM cells are elevated in the skin of SCT patients with cGVHD. Shown are the results of flow cytometric analysis of fresh lymphocytes isolated from 4-mm skin-punch biopsies (A) Representative surface expression of CD4-ECD and CD8-AmCyan, CD45RA-AF700 and CCR7-FITC, and CXCR3-PE on CD3-APC-Cy7⁺ T cells. (B-C) CD4 and CD8 (B), naive (NA, CD45RA⁺CCR7⁺), EM (CD45RA⁻CCR7⁺), central memory (CM, CD45RA⁻CCR7⁺), and EM RA⁺ (RA⁺CD45RA⁺CCR7⁻) expression (C), and CXCR3⁺ expression (D) by CD3⁺ T cells in control skin (n = 10) and in patients with cGVHD (n = 9). Data are expressed as the median frequency of CD3⁺ T cells with bars illustrating the interquartile range (B-C) and as the mean frequency with bars illustrating SD (D). *P < .05 and **P < .005 by the Mann-Whitney U test; elevated CXCR3⁺ expression was confirmed by 1-way t test.