DLBCL cells express and secrete gal-3. (A-C) Tissue array of 259 primary DLBCL tissue sections was analyzed for gal-3 expression by immunohistochemistry. (A) Low-power magnification of a portion of the tissue array stained for gal-3. (B) DLBCL tissues that showed cytoplasmic staining of tumor cells were scored as gal-3⁺. (C) Samples in which stromal cells expressed gal-3 but no gal-3 expression was detected in tumor cells were scored as gal-3⁻. SUDHL-6 and SUDHL-9 cell lines derived from patients with DLBCL were analyzed for total gal-3 expression by immunoblot (D) and gal-3 cell-surface expression by flow cytometry (E). For panel E, cells were fixed before staining to retain cell-surface gal-3.