TCRβ-chain repertoire of clonal CD3+ T cells determined by next-generation sequencing. Expanded T-cell clones were present in the fetal and infant gut in contrast to their absence in thymus, mesenteric lymph nodes (LN), and blood. (A) Scatterplot displaying the percentage of recovered reads per clone (dot) for each tissue or blood sample for patient 7 (fetus), patient 11 (term infant), and the adult peripheral blood control. The clonal distribution of the adult T cells was comparable to previously published data.17 Samples were adjusted for cell input. A cutoff based on a relative contribution of a T-cell clone of > 0.14% to the total T-cell population was used to identify expanded clones and allowed for comparison of tissues and patients. (B) Pie chart showing the distribution of the T-cell clones present in the gut of patient 7 over all the compartments, including gut, lymph nodes, thymus, spleen, and combinations. The T-cell clones were clearly compartmentalized with a lack of distribution over the other tissues.
