Evolved clone differences between CD45^lowCD10⁺CD38⁺ and CD45^lowCD10⁺CD38⁻ sorts of patient 23. (A) Flow cytometric plots illustrating CD45 and CD10 (left) and forward scatter (FSC) and CD38 expression (right) on bone marrow mononuclear cells from patient 23. Gate on plot at left indicates CD45^−/lowCD10⁺ “B-ALL” cells. Gates on plot at right indicates malignant 1 and 2 populations (based on the level of CD38 expression) within B-ALL cells identified at left. For this patient, an independent sort was performed to separate another malignant 1 and malignant 2 population based on CD45⁻CD10⁺ and CD45^lowCD10⁺. (B) Fold enrichment (compared with unsorted) of the frequency of each evolved clonotype in the 2 malignant populations, with the x-axis showing enrichment (compared with unsorted) in the CD45^−/lowCD10⁺CD38⁺ population and the y-axis showing enrichment (compared with unsorted) in the CD45^−/lowCD10⁺CD38⁻ population, with each point representing a malignant clone (index/evolved). (C) Fold enrichment (compared with unsorted) of the frequency of each of the evolved clonotypes in each of the 2 malignant populations, with the x-axis showing enrichment (compared with unsorted) in the CD45⁻CD10⁺ population and the y-axis showing enrichment (compared with unsorted) in the CD45^lowCD10⁺ population, with each point representing a malignant clone (index/evolved).