FANCL and β-catenin interaction may be facilitated by FANCA and FANCG. (A) Immunoprecipitation studies from cells transfected with cDNAs for human β-catenin and FANCL. (B) Top panel: immunoblot showing expression of C-terminal V5-tagged FANCA, C, and G proteins. Middle panel: immunoprecipitation studies from cells transfected with β-catenin and various V5-tagged FANC proteins as indicated. Bottom panel: reciprocal immunoprecipitation using V5 antibody then probed for β-catenin. At least 3 independent experiments were carried out for these studies. (C) Immunoprecipitation assays were also performed with untagged FANCC with the indicated isotype IgG controls. (D) MEFs from WT mouse and Fancc-deficient MEFs corrected by Fancc cDNA expression serve as control cells. Cells were treated with mitomycin C for 4 days at 62.5 ng/mL and whole cell extracts were analyzed for β-catenin expression. Four independent experiments were performed and shown is a representative result. The data are summarized as mean relative expression to untreated cells (no MMC) for each MEF cell line ± SEM and their corresponding P values. We probed for actin as a loading control for these experiments.