Increased percentages of NKT17 cells in Th-POK–deficient mice. (A) Plots depict flow cytometric analyses of electronically gated Th-POKhd/hd and control splenic Vα14i NKT cells stained for NK1.1 and (top row) CD196, (middle row) CD121a, and (bottom row) CD103. (B) Plots depict flow cytometric analyses of Th-POK+/+, Th-POKhd/+, and Th-POKhd/hd liver Vα14i NKT cells stained for expression of NK1.1 and CD122. (C) Scatter plot displaying the numbers of NKT17 cells in the livers of littermate or age-matched pairs of Th-POKhd/hd and Th-POKhd/+ mice (n = 9). Each pair is represented by a unique symbol. The P value was calculated using a Student t test on the log-transformed values. (D) Plots depict IL-17 and NK1.1 expression (left set) or IL-17 and RORγT expression (right set) in electronically gated splenic Vα14i NKT cells from Th-POK+/+, Th-POKhd/+, and Th-POKhd/hd mice that were challenged with 2 μg of αGalCer, as well as a Th-POK+/+ mock-injected animal. Mice were injected 2.5 hours before harvesting of organs. All data are representative of at least 2 separate experiments and a total of at least 3 sets of Th-POKhd/hd and control mice.
