Reduced memory B cells in blood of CGD patients. PBMCs of CGD patients and HDs were stained with antibodies against CD3, CD10, CD19, CD21, and CD27 for FACS analysis. Gating on CD19⁺/CD3⁻ cells established the B-cell population. The mean percentages of each B-cell subset, based on a (A) 3- or (B) 5-subset definition, were calculated within the B-cell population (left panel), and white blood cell counts and percent lymphocyte from complete blood counts were used to calculate absolute counts for each subset (right panel). *P < .05. **P < .001. ***P < .0001. Horizontal bars represent medians. †Subset also includes tissue-like (CD21^lo/CD27⁻) memory B cells, but these B cells represented < 3% of total B cells for both groups.