Analysis of the effects (mean ± SEM) of iPTH treatment in PPR^{T cells−/−} and control mice of 6 weeks of age. (A) Effects of iPTH on the relative frequency of BM LSK cells. (B-C) Effect of iPTH on the relative frequency of ST-HSPCs/MPP and LT-HSPCs. (D-F) Effect of iPTH on peripheral blood cell expansion after primary competitive repopulation. The percentages of CD11b⁺, GR-1⁺, and B220⁺ cells in the peripheral blood of untreated WT recipient mice are shown. Recipient mice received CD45.2⁺ BM donor cells from control or PPR^{T cells−/−} mice (treated with vehicle or iPTH) mixed in a 1:2 ratio with CD45.1⁺ competitor BM cells from untreated WT mice. (G-J) Kaplan-Meier survival analysis of WT mice transplanted with limiting number of BM cells derived from PPR^{T cells−/−} and control (PPR^fl/fl) mice. (G-H) Donor mice were untreated. Recipient mice were treated with vehicle or iPTH for 4 weeks. (I-J) Donor mice were treated with vehicle or iPTH for 4 weeks. Recipient mice were untreated. n = 10-15 in each group. *P < .05 versus the corresponding vehicle-treated group.