Lack of osteoblastic N-cadherin results in increased bony trabecular structures. (A) Representative image of a frozen section from a Col1-cre, Z/EG mouse. eGFP resulting from Cre recombination is green and highlighted by the arrow. The white dotted line indicates the endosteal surface with bone to the left and marrow to the right. (B-C) Quantitative RT-PCR analysis of N-cadherin mRNA expression. (B) Cells isolated from adult long bones. (C) Cells isolated from neonate calvaria. (D) Representative images of paraffin sections from WT (top panels) or OB-NCadh mice (bottom panels) that are untreated (left panels) or treated with PTH (middle and right panels) immunohistochemically stained for N-cadherin in which N-cadherin⁺ cells are pink. Images obtained using a 60× objective. (E) Representative micro-CT scans of trabecular bone at the metaphysis of the femur (top) or tibia (bottom). (F) Representative micro-CT scans of the femur and tibia. (G) Analysis of micro-CT scans showing trabecular BV/TV, trabecular number, trabecular connectivity density, and trabecular spacing. *P < .05. n = 6 to 12 male mice per group.