Lack of osteoblastic N-cadherin does not alter steady-state hematopoiesis or functional HSPCs in aged mice. (A) Total marrow cellularity as determined by counting flushed marrow cells (n = 3-6 mice per group). (B) Spleen weight in grams (n = 3-6 mice per group). (C) Frequency of the LSK, MPP/ST-HSC, and LT-HSC populations as defined in Figure 2. N = 2 WT, 6 OB-NCadh 1-year-old mice. (D-F) Analysis of the peripheral blood after competitive transplantation shows the percentage of donor cells determined by cell surface expression of CD45.2 in the total peripheral blood cell population, CD11b⁺ population (D), B220⁺ population (E), and CD3e⁺ population (F). Recipient mice were analyzed at 4 to 22 weeks after transplantation of donor and competitor cells. *P < .05 at 22 weeks only by Bonferroni posttest analysis. No significant differences were noted by 2-way ANOVA with repeated-measures analysis using the Bonferroni posttest after transplantation of donor and competitor cells. n = 3 WT and 4 OB-NCAD donors per genotype, 13 to 20 recipients per donor group (4 or 5 recipients for each individual donor). (G-I) Hematopoietic recovery after sublethal (6.5 Gy) total body irradiation: hematocrit (G), WBCs (H), and platelets (I). No significant differences were noted between genotypes by 2-way ANOVA. n = 3 to 5 mice per genotype per time point.