PTH treatment (3 times a day) increases trabecular bone volume and osteoclastic cells. (A-B) Representative images of H&E-stained paraffin sections from the distal femur of (A) VEH-treated and (B) PTH-treated mice. Images were obtained using a 4× objective. (C) Representative images of TRAP-stained paraffin sections from VEH-treated (top panel) and PTH-treated (bottom panel) mice. Sections were counterstained with hematoxylin. Images were obtained using a 20× objective. (D) Quantification of TRAP⁺ cells from paraffin sections represented by panel C. (E-J) Micro-CT analysis of trabecular bone from the femur of VEH- and PTH-treated mice, including (E) total volume, (F) bone volume/total volume, (G) connectivity density, (H) trabecular spacing, (I) trabecular thickness, and (J) trabecular number. (K) Representative micro-CT images of trabecular bone from the femur of VEH- or PTH-treated mice. *P < .05. **P < .01. ***P < .001. n = 5 or 6 per treatment group.