Granzyme B and perforin in CD8-LV TCR-transduced T cells. PBMCs were treated as described in Figure 4. CD8-LV-TCR-T58 or VSV-G-LV-TCR-T58–transduced PBMCs (MOI = 2) were incubated with Mel-624.38 (E:T = 2:1) in the presence of GolgiPlug. After 4 hours, cells were surface stained with anti-CD8 and anti-mCβ mAbs to determine the total number of effector cells (CD8⁺/TCR-T58⁺), and intracellularly stained with specific mAbs to determine the percentage of GrB (A) or perforin (B) expressing cells. Data are from 5 different donors. Individual symbols represent individual donors to visualize trends per donor. Bars represent median values (*P < .05; **P < .01).