Impaired NK cell development in BM and periphery of SHIP^−/− mice. (A) Percentages of NKPs (CD122⁺NK1.1⁻CD3⁻CD11b⁻), iNK cells (CD122⁺NK1.1⁺CD3⁻CD11b⁻), and mNK cells (CD122⁺NK1.1⁺CD3⁻CD11b⁺) among bone marrow lymphocytes derived from littermate SHIP^+/+ (white circles), SHIP^+/− (gray circles), and SHIP^−/− (black circles) mice. Data are pooled from at least 5 independent experiments, and each dot represents data obtained from one mouse; horizontal lines indicate the mean. (B) Representative staining in NK cells (NK1.1⁺CD3⁻) expressing DN (CD27⁻CD11b⁻), CD27 SP (CD11b⁻CD27⁺), DP (CD27⁺CD11b⁺), and CD11b SP (CD27⁻CD11b⁺) populations in spleens from SHIP^+/+, SHIP^+/−, and SHIP^−/− mice. (C) Percentages in NK cells (NK1.1⁺CD3⁻) being DN, CD27 SP, DP, and CD11b SP populations in the indicated organs. Data are representative of at least 8 independent experiments. Data represents means ± SD. (D left panel) Frequency of bone marrow PrePro NK cells (Lineage⁻, Sca-1⁺, CD117⁻, CD135⁻, CD127⁺). Data are pooled from 3 independent experiments. (Right panel) Total number of bone marrow cells.