NK cell number is rescued in chimeric mice. (A) Diagram of chimera generation. SHIP^+/− or SHIP^−/− (CD45.1⁺) bone marrow cells were transplanted into lethally irradiated WT (CD45.2⁺) recipients and analyzed 4 to 9 weeks after reconstitution. (B) Percentage and total NK cells (NK1.1⁺CD3⁻ in the lymphocyte gate) present in the indicated organs from SHIP^+/− chimera (gray circles) and SHIP^−/− chimera (black circles) mice. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. (C) Percentage of donor contributions of NKPs (CD122⁺NK1.1⁻CD3⁻CD11b⁻), iNK cells (CD122⁺NK1.1⁺CD3⁻CD11b⁻), and mNK cells (CD122⁺NK1.1⁺CD3⁻CD11b⁺) among bone marrow lymphocytes derived from SHIP^+/− chimeras (gray circles) and SHIP^−/− chimeras (black circles) mice. Data are pooled from at least 5 independent experiments, and each symbol indicates an individual mouse; horizontal lines indicate the mean. (D) Percentages in NK cells (NK1.1⁺CD3⁻ in the lymphocyte gate) expressing DN (CD27⁻CD11b⁻), CD27 SP (CD11b⁻CD27⁺), DP (CD27⁺CD11b⁺), and CD11b SP (CD27⁻CD11b⁺) populations in the indicated organs derived from SHIP^+/− chimera (gray bars) and SHIP^−/− chimera (black bars) mice. Data are pooled from at least 5 independent experiments. Error bars indicate SEM.