SHIP-1 regulates NK cell final stage of maturation intrinsically. (A) Diagram of mixed chimera generation. SHIP^+/− or SHIP^−/− (CD45.1⁺) bone marrow cells were isolated and mixed at an equal ratio with wild-type (WT) competitor (CD45.2⁺) bone marrow cells before transferring into lethally irradiated WT (CD45.2⁺) recipients and analyzed 8 to 10 weeks after reconstitution. (B) Percentages and total NK cells (NK1.1⁺CD3⁻ in the lymphocyte gate) present in the indicated organs from SHIP^+/− mixed chimeras (gray circles for SHIP^+/− and gray triangles for competitor) and SHIP^−/− mixed chimeras (black circles for SHIP^−/− and black triangles for competitor). (C) Percentages in NK cells (NK1.1⁺CD3⁻ in the lymphocyte gate) expressing DN (CD27⁻CD11b⁻), CD27 SP (CD11b⁻CD27⁺), DP (CD27⁺CD11b⁺), and CD11b SP (CD27⁻CD11b⁺) populations in the indicated organs derived from SHIP^−/− mixed chimeras (black bars for SHIP^−/− and striped bars for competitor). Data are pooled from at least 5 independent experiments. Error bars indicate SEM.