Impaired cytokine receptor–mediated response from SHIP-1–deficient NK cells is cell intrinsic. (A-B) Intracellular IFN-γ and CD107α in NK cell populations (NK1.1⁺CD3⁻ or DX5⁺CD3⁻ in the lymphocyte gate) from total splenocytes incubated with antibody-coated plates using anti-Ly49H, anti-NK1.1, and anti-NKG2D or with a mix of IL-12/IL-18 and PMA and ionomycin (P/I) for 6 hours are accessed after gating on donor cells (CD45.1⁺ for SHIP^−/−; CD45.1⁻ for competitor). (A) Mixed chimeras are pooled from at least 4 experiments. (B) Poly(I:C) primed mixed chimeras are pooled from at least 3 experiments. Error bars indicate SEM.