American Society of Hematology

Figure 1

$Figure 1. A3G expression correlates with DSB repair efficiency and survival after IR. (A) Western blot showing A3G protein level in lymphoma and leukemia cell lines. α-tubulin was used as a loading control. Molecular weights (kDa) are indicated on the left. (B) Cells were exposed to γ-radiation (4 Gy) and stained after 8 hours with anti-A3G and anti–γ-H2AX antibodies. The fraction of cells containing DSBs according to γ-H2AX staining is plotted against relative A3G expression measured in panel A. Values represent mean ± SD from 3 independent experiments and at least 10 different fields for each cell line analyzed. (C) Cells were exposed to γ-radiation and stained after 24 hours with anti-A3G and anti–γ-H2AX antibodies. Nuclei were counterstained with DAPI (original magnification ×630). CL indicates cutaneous lymphoma; BL, Burkitt lymphoma; DLBCL, diffuse large B-cell lymphoma; T-LL, T lymphoblastic leukemia; and ALL, acute lymphocytic leukemia. (D) Cells were exposed to γ-radiation (4 Gy) or mock and counted after 7 days. Cell survival was calculated as percentage of trypan blue-negative irradiated cells out of nonirradiated cells. Values represent mean ± SD from 2 independent experiments.$

A3G expression correlates with DSB repair efficiency and survival after IR. (A) Western blot showing A3G protein level in lymphoma and leukemia cell lines. α-tubulin was used as a loading control. Molecular weights (kDa) are indicated on the left. (B) Cells were exposed to γ-radiation (4 Gy) and stained after 8 hours with anti-A3G and anti–γ-H2AX antibodies. The fraction of cells containing DSBs according to γ-H2AX staining is plotted against relative A3G expression measured in panel A. Values represent mean ± SD from 3 independent experiments and at least 10 different fields for each cell line analyzed. (C) Cells were exposed to γ-radiation and stained after 24 hours with anti-A3G and anti–γ-H2AX antibodies. Nuclei were counterstained with DAPI (original magnification ×630). CL indicates cutaneous lymphoma; BL, Burkitt lymphoma; DLBCL, diffuse large B-cell lymphoma; T-LL, T lymphoblastic leukemia; and ALL, acute lymphocytic leukemia. (D) Cells were exposed to γ-radiation (4 Gy) or mock and counted after 7 days. Cell survival was calculated as percentage of trypan blue-negative irradiated cells out of nonirradiated cells. Values represent mean ± SD from 2 independent experiments.

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