Transgenic IL-7–expression during LN reconstruction after avascular transplantation. LNs from IL-7–CrexR26-EYFP donor mice were transplanted under the kidney capsule of C57BL/6 mice and harvested 8 weeks after transplantation. (A) Mosaic scan of a regenerated LN (tLN) developing on the kidney (Ki; blue represents B220; red, Pdpn; and green, EYFP). Scale bar represents 200 μm. (B) Three-dimensional reconstruction of subcapsular sinus region (red represents LyveI; and green, EYFP). Scale bar represents 50 μm. Arrows indicate transgene-positive LECs. (C) Three-dimensional reconstruction of T-cell zone region (red represents Pdpn; and green, EYFP). Scale bar represents 50 μm. Arrows indicate transgene-positive FRCs. (D) Lymphatic vessel connecting tLN with surrounding tissue (blue represents Pdpn; red, LyveI; and green, EYFP). Scale bar represents 100 μm. (E) Three-dimensional rendering of boxed region in panel D showing lymph vessel connection to the SCS (red represents LyveI; and green, EYFP). Scale bar represents 20 μm.