FRATN-expressing cells are selectively lost from culture. (A-B) Graphs showing the percentage of (A) hCD2T⁺ leukemic MLL-ENL cells (data are representative of 2 independent experiments) and (B) hCD2T⁺ preleukemic MLL-AF9 cells, at indicated time points in liquid culture, after transduction with control and FRATN-expressing retroviral vectors. Data are representative of 3 independent experiments. Error bars represent SD of mean values (n = 3). (C) Leukemic MLL-ENL cells were transduced with active β-catenin or control vectors; and 48 hours later, hCD4T⁺ cells were purified by MACS. hCD4T⁺ cells were then transduced with the FRATN-expressing or control pMSCV-IRES-hCD2T retroviral vectors. Graph showing the percentage of control or FRATN-transduced hCD2T⁺ leukemic MLL-ENL cells, with or without active β-catenin, at indicated time points in liquid culture. Error bars represent SD of mean values (n = 3). Data are representative of 2 independent experiments.