TF expression in sickle cell mice. (A) TF mRNA expression and (B) procoagulant activity (PCA) in organs of WT (open bar; n = 9) and BERK mice (filled bar; n = 8). K indicates kidney; Lu, lung; H, heart; Li, liver; and S, spleen. Asterisks directly above the bars indicate statistically significant difference compared with WT controls: **P < .01 and ***P < .001. (C) TF-dependent PCA activity in the spleens of WT (open bar) and BERK mice (filled bar). *P < .05. Immunocytochemistry (D-E) or immunofluorescence (F-G) staining demonstrates TF-positive leukocytes with nuclear morphology resembling monocytes (D,F) or neutrophils (E,G). TF is shown as brown staining (top panels) or green staining (bottom panels). Nuclei are stained blue. (H) TF activity of the leukocytes isolated from the same volume of blood obtained from WT (n = 13) or BERK (n = 10) mice. **P < .01. (I) TF activity of the microparticles (MP TF) isolated from the plasma of WT (open bar; n = 6) and BERK (filled bar; n = 15) mice. (J) MP TF activity in TW^AA (open bar; n = 13) and TW^SS (filled bar; n = 17) mice.