Role of endothelial cell TF in the activation of coagulation, inflammation, and endothelial cell activation in sickle cell mice. (A) Representative gel demonstrating the analysis of different hemoglobin types by cellulose acetate electrophoresis in TF^flox/flox,Tie-2 Cre mice transplanted with bone marrow from BERK mice (lines 1-3 and 9) or WT mice (lines 4 and 6-8). Upper and lower bands correspond to human sickle hemoglobin and normal mouse hemoglobin. Lines 5 and 10 contain positive controls (con) for both forms of hemoglobin. Line 11 shows the example of unsuccessful transplantation of bone marrow from BERK into TF^flox/flox,Tie-2 Cre mouse. Mice like this were excluded from experiments. A vertical line has been added to indicate 2 separate gels. (B-H) Plasma levels of TAT, IL-6, IL-18, SAP, and sVCAM-1 as well as tissue levels of MPO in lung and liver were analyzed in WT (open bar) and sickle cell mice (filled bar) with (+) or without (−) TF gene deletion in endothelial cells (EC TF Δ; n = 9-14). Asterisks directly above the bars indicate statistically significant difference compared with WT^BM controls within the same treatment: *P < .05; **P < .01; and ***P < .001.