The MAPK/ERK but not the PI3K pathways are involved in the increase by basophils of IL-17⁺/IFN-γ⁻ and IL-17⁺/ IFN-γ⁺ T cellsin T_EM and T_CM. T_EM stimulated by IL-2 and T_CM stimulated by anti-CD3/28–coated beads were cultured in the presence of IL-33 with or without basophils and in the absence or presence of PI3K inhibitor (LY294002 at 2μM) or ERK1/2 inhibitor (PD98059 at 20μM). Shown is intracytoplasmic staining for IL-17 and IFN-γ expression after PMA ionomycin restimulation for 6 hours. Data represent means ± SEM for 6 donors. *,^#P < .05; **,^##P < .01; ***P < .001 (* indicates cultures ± inhibitors and #, cultures ± basophils).