Tyrosine phosphoproteome analysis of rhodocytin-stimulated platelets. (A) Phosphoprotein staining of a 10% SDS-PAGE gel showing phosphorylated proteins obtained after 4G10 antiphosphotyrosine immunoprecipitation of basal and rhodocytin-stimulated platelets. (B) Total protein staining of the same gel showing all the proteins immunoprecipitated with the 4G10 antibody in basal and rhodocytin-stimulated platelets. Indicated bands were excised from the gel and analyzed by MS. Proteins identified are reported in supplemental Table 1. Basal indicates basal platelets; Rhod, platelets stimulated with rhodocytin (300nM, 5 minutes); and IP, immunoprecipitation.