High-resolution 2D-DIGE proteome analysis of rhodocytin-activated platelets. Proteins were labeled with the corresponding Cy-dyes (“Methods”) and separated using isoelectric focusing (pH range 4-7, 24 cm) and 10% SDS-PAGE gels. (A) Representative image of the 2D-DIGE analysis where the fluorescence emission from Cy3 and Cy5 dyes is superimposed. Green color spots are up-regulated in rhodocytin-stimulated platelets, whereas red-orange color spots are down-regulated. The array of altered spots that contained the protein pleckstrin is indicated. (B) Representative image of the analysis in gray scale. Spots differentially regulated comparing nonstimulated and rhodocytin-activated platelets are indicated. Further information about protein identifications can be found in supplemental Table 2.