Dok-2 interaction with SHIP-1 is increased after CLEC-2 platelet stimulation. Dok-2 and SHIP-1 interaction was studied using coimmunoprecipitation and time-course experiments. Proteins were separated using 4%-12% NuPAGE Bis-Tris gels before Western blotting. (A) Representative images. (B) Densitometry graphs showing average band intensities and statistics. *P < .05. Basal indicates basal platelets; Rhod, platelets stimulated with rhodocytin (300nM) for 30 seconds, 2 minutes, and 5 minutes; IP, immunoprecipitation; and IB, immunoblot. Experiments were performed at least 4 times.