Comparative analysis of GPVI and CLEC-2 signalosomes. (A) Venn diagram showing comparative data on proteins identified by GPVI and CLEC-2 signaling proteomic analyses. (B) Venn diagram showing comparative data on signaling proteins identified by GPVI and CLEC-2 tyrosine phosphoproteome analyses. (C) Tyrosine phosphoproteome profile comparing platelets stimulated with rhodocytin and with CRP. Immunoblot with 4G10 is shown to visualize tyrosine-phosphorylated proteins after antiphosphotyrosine immunoprecipitation with the 4G10 antibody and protein separation on a 4%-12% NuPAGE Bis-Tris mini gel. B indicates basal platelets; Rhod, platelets stimulated with rhodocytin (300nM, 5 minutes); CRP, platelets stimulated with collagen-related peptide (10 μg/mL, 90 seconds); IP, immunoprecipitation; and IB, immunoblot. Platelet stimulations were in the presence (+) and absence (−) of inhibitors of secondary mediators (apyrase and indomethacin).