rhADAMTS13 reduces myocardial apoptosis after transient LAD occlusion. (A) Representative immunofluorescent DAPI (blue), TUNEL (green), and Gr-1 (red) stainings of sections from the ischemic area in the hearts of mice that received rADAMTS13 or vehicle. Apoptosis was absent in nonischemic areas (not shown). The higher magnification of the double stain (Gr-1/TUNEL) shows that majority of TUNEL-positive cells are not Gr-1–positive, that is, they are green rather than yellow (arrows). (B) The apoptotic index of both groups was determined by calculating the percentage of apoptotic cells (measured in 3 different view fields per section, 4 sections per mouse with 3 mice per group, ***P < .001). Scale bar = 50 μm.