Improvement of thymic epithelium in Rag2^R229Q mice 2 months after anti-CD3ε mAb administration. (A) Representative H&E staining of a thymus obtained from PBS and anti-CD3ε mAb-treated RAG2^R229Q newborns (original magnification, ×20). (Right panel) Graphic representation of M/C ratio in the 2 groups (PBS n = 8, anti-CD3ε n = 9); ***P = .0003. (B) CK5, CK8, UEA-1, and AIRE immunohistochemistry of thymus from mice treated as indicated (original magnification, ×20). (C) Quantitative analysis of AIRE⁺ cells obtained as described in “Analysis of the ratio between medullary and cortical area and tissue-infiltration score” in PBS (n = 6) and anti-CD3ε mAb (n = 10) mice. (D) Absolute number of different epithelial cell subsets obtained after enzymatic digestion of thymi from PBS and anti-CD3ε mAb mice (n = 5); **P = .0079.