Figure 4
Comparison of PDI secretion from WT and β3−/− mouse platelets. (A) Platelets from wild-type (wt) and β3−/− mice (1 × 109 platelets/100 μL) were incubated with thrombin for 5 minutes, and the supernatant and pellet were separated. The pellet was solubilized with 0.1 mL of SDS lysis buffer. Washed human platelets (1 × 109 platelets/mL) were lysed with 0.1 mL of SDS lysis buffer. Washed human platelets (10 μL), the platelet releasates (S; 5 μL), and platelet lysates (P; 5 μL) of WT and β3−/− mouse platelets (5 μL) were electrophoresed in an SDS-PAGE gel and immunoblotted with rabbit polyclonal anti-PDI antibodies or nonimmune rabbit IgG. hplt indicates human platelets.
