PDI accumulation and platelet thrombus formation in β3^−/− mice. Noninhibitory rabbit anti-PDI antibody conjugated to Alexa 488 (0.5 μg/g body weight) and antifibrin-specific antibody conjugated to Alexa 647 (0.3 μg/g body weight) were infused into a wild-type (wt) or β3^−/− mouse 5 minutes before laser-induced arteriolar wall injury. (A) Representative images of the fluorescence signals associated with PDI (green) and fibrin (red) are shown over a course of 180 seconds after vessel injury within the context of the bright-field microvascular histology. The median integrated fluorescence associated with PDI fluorescence (F_PDI) and fibrin fluorescence (F_fibrin) after infusion of anti-PDI antibodies (B) and antifibrin antibodies (C) in 3 WT mice (n = 28 thrombi) and 3 β3^−/− mice (n = 25 thrombi) is presented over a course of 250 seconds after vessel wall injury.