ELISPOT analysis for IFN-γ and granzyme B of several NPM1-derived peptides in an NPM1^mut AML patient. ELISPOT assays for the release of IFN γ (A) and granzyme B (B) were performed after stimulation with several NPM1-derived peptides. Stimulation with IMP served as a positive control and no peptide stimulation as a negative control. Peptide-specific T-cell activity was measured by IFN release, whereas granzyme B secretion indicated the lytic potential of the T cells. This patient showed a significant increase in frequency for the epitopes #1 and #3 derived from the mutational region of NPM1.