OL analysis for CD4 and/or CD8 recognition. All OLs were analyzed by ELISPOT with PBMCs from 8 HVs individually for granzyme B (A). NPM1#1 CD8⁺ T cells were assessed for IFN-γ (B) and granzyme B (C) secretion activity in settings of coculture with HLA-DR*0701/binding OL mixture of 5-8. Secretion of CD8⁺ T cells was diminished by specifically blocking the HLA-A2 receptor as assessed by granzyme B ELISPOT (D).