Skin injury induces the rapid accumulation of inflammatory CCR2⁺Ly6C⁺ monocytes/macrophages. (A) Representative FACS analysis of single-cell suspensions of wound tissue from CCR2^fl/D mice at different time points after injury. F4/80⁺CD11b⁺ cells were gated and analyzed for expression of eGFP and Ly6C. (B) Fractions P1-P6 were gated and quantified (n = 6-10 wounds on 2-5 mice per time point). (C) Top, SSC^lowCD11b⁺CD115⁺ blood monocytes were gated and FACS sorted for qRT-PCR analysis. Bottom, qRT-PCR analysis of selected genes in F4/80⁺CD11b⁺ macrophages isolated from wound tissue of control mice at different time points after injury normalized to gene expression in SSC^lowCD11b⁺CD115⁺ blood monocytes (n = 4-8 wounds on 2-4 mice per time point). (D) qRT-PCR analysis of selected genes in F4/80⁺CD11b⁺ macrophages gated for P1-P6 fractions isolated from wound tissue in CCR2^fl/D mice at different time points after injury and normalized to gene expression in SSC^lowCD11b⁺CD115⁺ blood monocytes. Each dot represents 1 mouse (tissues of 4 wounds per mouse were pooled). A minimum of 2 independent experiments were performed and data are expressed as means ± SD. *P < .05; **P < .01; ***P < .001.